Bio-Deuteration Laboratory:

Neutron scattering studies are greatly enhanced by the design and production of specific, random and uniform hydrogen/deuterium labeled biological macromolecules that permit selected parts of macromolecular structures to be highlighted and analyzed in situ. CSMB has established a Bio-Deuteration Laboratory for in vivo production of hydrogen/deuterium-labeled bio-macromolecules for research at HFIR and SNS. The Bio-Deuteration Laboratory provides neutron users with access to facilities and expertise for cloning, protein expression, purification and characterization of deuterium-labeled biological macromolecules and trains visiting researchers in the use and application of these techniques. These capabilities will allow our facility users to design and produce proteins, complexes and macromolecular assemblies that are optimized for neutron scattering analysis and will enable these data to be used to construct biologically meaningful models of protein complexes, assemblies and hierarchical structures.

The Bio-Deuteration Laboratory is operated to support the structural biology community. Contact us at 865.574.0824 for further information. Or, if you would like to become a user, please submit your proposal here.